Table II.
Intra- and inter-day accuracy and precision of QCs, LLOQ and ULOQ samples in plasma.
| Nominal concentration, µg/ml | Intra-day a (n=5) | RSD, % | RE, % | Inter-daya (n=5, 6 daysb) | RSD, % | RE, % |
|---|---|---|---|---|---|---|
| FTFSLDTSK | ||||||
| 5 | 4.85±0.59 | 12.20 | −2.95 | 5.02±0.81 | 16.18 | 0.41 |
| 10 | 9.36±1.02 | 10.84 | −6.43 | 9.69±1.28 | 13.18 | −3.09 |
| 50 | 47.74±3.08 | 6.45 | −4.52 | 51.43±5.92 | 11.51 | 2.86 |
| 200 | 196.09±7.08 | 3.61 | −1.95 | 192.79±16.41 | 8.51 | −3.60 |
| 400 | 402.44±18.24 | 4.53 | 0.61 | 370.10±32.57 | 8.80 | −7.48 |
| VLIYFTSSLHSGVPSR | ||||||
| 5 | 5.10±0.70 | 13.70 | 1.92 | 5.12±0.74 | 14.39 | 2.47 |
| 10 | 9.70±0.34 | 3.48 | −3.02 | 9.42±1.212 | 12.93 | −5.76 |
| 50 | 51.10±4.48 | 8.76 | 2.20 | 48.86±4.47 | 9.15 | −2.28 |
| 200 | 218.08±8.62 | 3.95 | 9.04 | 192.04±21.09 | 10.98 | −3.98 |
| 400 | 394.60±6.44 | 1.63 | −1.35 | 375.16±27.13 | 7.23 | −6.21 |
| STAYLQMNSLR | ||||||
| 5 | 4.90±0.58 | 11.80 | −2.04 | 5.09±0.96 | 18.84 | 1.71 |
| 10 | 9.66±1.05 | 10.85 | −3.37 | 10.04±1.46 | 14.53 | 0.44 |
| 50 | 52.76±4.77 | 9.04 | 5.52 | 49.81±5.35 | 10.73 | −0.38 |
| 200 | 204.29±13.40 | 6.56 | 2.15 | 192.66±16.84 | 8.74 | −3.67 |
| 400 | 415.61±24.71 | 5.95 | 3.90 | 399.65±31.18 | 7.80 | −0.09 |
a
Data are shown as mean ± SD.
b
Independent validation days for accuracy and precision. QC, quality control; LLOQ, lower limit of quantitation; ULOQ, upper limit of quantitation; RE, relative error; RSD, relative standard deviation.