Table III.
Stability results of selected peptides (FTFSLDTSK, VLIYFTSSLHSGVPSR, STAYLQMNSLR) in plasma in different conditions.
| Nominal concentration, µg/ml | FTFSLDTSKa | RSD, % | RE, % | VLIYFTSSLHSGVPSRa | RSD, % | RE, % | STAYLQMNSLRa | RSD, % | RE, % |
|---|---|---|---|---|---|---|---|---|---|
| Short-term (25°C for 6 h) | |||||||||
| 10 | 9.74±1.11 | 11.35 | −2.57 | 9.84±1.25 | 12.69 | −1.62 | 9.70±1.18 | 12.21 | −3.00 |
| 50 | 49.75±2.82 | 5.67 | −0.50 | 47.86±1.27 | 2.65 | −4.29 | 51.08±5.24 | 10.27 | 2.15 |
| 200 | 186.33±3.38 | 1.81 | −6.84 | 189.08±7.56 | 4.00 | −5.46 | 219.35±14.93 | 6.81 | 9.67 |
| Auto-sampler (4°C for 72 h) | |||||||||
| 10 | 9.20±1.14 | 12.36 | −8.04 | 11.21±0.81 | 7.18 | 12.10 | 9.81±1.40 | 14.30 | −1.87 |
| 50 | 49.80±4.26 | 8.56 | −0.40 | 48.44±3.79 | 7.83 | −3.12 | 55.81±4.79 | 8.58 | 11.63 |
| 200 | 192.51±7.40 | 3.84 | −3.75 | 185.69±5.06 | 2.72 | −7.16 | 213.34±4.50 | 2.11 | 6.67 |
| Freeze thaw (−80-25°C for 3 cycles) | |||||||||
| 10 | 9.17±1.18 | 12.86 | −8.26 | 9.18±0.59 | 6.43 | −8.17 | 9.35±0.98 | 10.49 | −6.51 |
| 50 | 47.21±5.71 | 12.09 | −5.59 | 46.44±1.67 | 3.61 | −7.12 | 45.63±3.46 | 7.57 | −8.74 |
| 200 | 190.14±11.93 | 6.27 | −4.93 | 191.43±4.24 | 2.21 | −4.28 | 189.56±9.47 | 4.99 | −5.22 |
| Long term (−80°C for 26 days) | |||||||||
| 10 | 10.03±0.98 | 9.78 | 0.27 | 8.79±0.76 | 8.62 | −12.08 | 9.31±1.11 | 11.94 | −6.90 |
| 50 | 48.81±3.68 | 7.55 | −2.37 | 44.88±4.72 | 10.52 | −10.24 | 47.86±4.78 | 9.99 | −4.29 |
| 200 | 191.94±7.10 | 3.70 | −4.03 | 191.56±6.63 | 3.46 | −4.22 | 189.65±14.77 | 7.79 | −5.18 |
a
Data are shown as mean ± SD. RSD, relative standard deviation; RE, relative error.