Table 2.
Oligonucleotides used in this study
| Name | Sequence |
|---|---|
| For isogenic psmα/psmδ deletion mutant | |
| psmαδ-att1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACAAATAATGTTGCACCCCAAAACATGGTCAT |
| psmαδ-rev1 | TGTCCCAGGCCCATGTAGAACCCTTACTAATTTGTTATCTATAGTGTAACTGGAAT |
| psmαδ-rev2 | TTCTACATGGGCCTGGGACATAATTCCTATC |
| psmαδ-att2 | GGGGACCACTTTGTACAAGAAAGCTGGGTTCATAAATGTGGCATCGTGATCTGTTTTAGAATAA |
| For isogenic psmε deletion mutant | |
| psmε-att1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTTTCAAAAAGATTTTTTACTACAAATATAATAGAATTAACA |
| psmε-rev1 | GAGGGGCGGAGTAGAGAATACACCTCCTATGTATTTGTTAACTAAATTATAGTATTGAAC |
| psmε-rev2 | TATTCTCTACTCCGCCCCTCCTGTTAGAGG |
| psmε-att1 | GGGGACCACTTTGTACAAGAAAGCTGGGTACCCTAGACCTGTTGAAAGTGGCATTATGA |
| For isogenic psmα start codon change mutant | |
| psmα-att1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACAAATAATGTTGCACCCCAAAACATGGTCAT |
| psmα-rev1 | AGGGAGGTCATTTAAATAGCAGATGTAATC |
| psmα-rev2 | ATTACATCTGCTATTTAAATGACCTCCCTT |
| psmα-att2 | GGGGACCACTTTGTACAAGAAAGCTGGGTTCATAAATGTGGCATCGTGATCTGTTTTAGAATAA |
| For isogenic psmδ deletion mutant | |
| psmδ-att1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACAAATAATGTTGCACCCCAAAACATGGTCAT |
| psmδ-rev1 | TGTCCCAGGCCCATGTAGAATCCTTTCAAAAGGGAAATGTATTTAGAGATG |
| psmδ-rev2 | TTCTACATGGGCCTGGGACATAATTCCTATC |
| psmδ-att2 | GGGGACCACTTTGTACAAGAAAGCTGGGTTCATAAATGTGGCATCGTGATCTGTTTTAGAATAA |