TABLE 3.
Efficiencies of marker transfer experiments
| Virus strain for:
|
Gene for PCR product | BDCRB concn (μM) | Plating efficiencya (%) | Recombinant strain | |
|---|---|---|---|---|---|
| Parental DNA | PCR product | ||||
| AD169 | wt (Towne) | UL89 | 2 | 0.070 | |
| AD169 | B11 (Towne) | UL89 | 2 | 0.50 | rT89E2-4 |
| D10 | wt (Towne) | UL56 | 20 | <0.003 | |
| D10 | C4 (Towne) | UL56 | 20 | 0.29 | rC4 |
| Towne | wt (Towne) | UL56 | 5 | <0.005 | |
| Towne | C4 (Towne) | UL56 | 5 | 5.4 | r56 |
a
HFF cells were cotransfected with the indicated sources of parental and mutated DNA (PCR product). Following transfections, stocks of progeny virus were grown and titers were determined. Five flasks (20,000 to 70,000 PFU per flask) were infected with this virus and incubated in the presence of the indicated concentrations of BDCRB. Five flasks also were infected in the absence of drug, using 1/10 the amount of virus. Plating efficiency is the percentage of plated recombination progeny which formed plaques under the indicated concentration of BDCRB as described in the text.