[Solution A] The buffer should be autoclaved after the preparation
| Reagent | Final concentration | Amount |
|---|---|---|
| KH2PO4 | 22 mM | 3 g |
| Na2HPO4 | 49 mM | 6 g |
| NaCl | 8.6 mM | 0.5 g |
| Tap water | Up to 100 mL | |
| Total | 100 mL |
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