Reagent | Final concentration | Amount |
---|---|---|
10 mM FeCl3 | 56 μM | 5.0 mL |
1 M MgSO4 | 2.3 mM | 2.0 mL |
50 mM MnCl2 | 56 μM | 1.0 mL |
Thiamine Hydrochloride | 270 μM | 80 mg |
Adenosine | 8.5 μM | 2 mg |
Thiamine | 8.6 μM | 2 mg |
Guanosine | 8.0 μM | 2 mg |
Cytosine | 20 μM | 2 mg |
D-biotin | 9.3 μM | 2 mg |
Deionized Water | 872 mL | |
Total | 880 mL |