FIG. 2.

Transfection-infection assays with pK26GFP. Vero cells seeded at 3.5 × 10⁴ cells/cm² in culture dishes were transfected approximately 16 h later with 5 μg each of plasmids pEGFP-N1 (A) or pK26GFP (B and C) (CellPhect; Pharmacia Upjohn). Twenty-four hours after transfection the cells were infected with either KOS (B) or K5ΔZ (C) at an MOI of 10 PFU/cell or were mock infected (A). Cells were visualized live 12 h postinfection with an Olympus BH-2 fluorescence microscope (×20 objective).