Fig. 4.

Measured vs true N1 copies per reaction as a function of sequence. True concentrations of each oligomer (measured via the N2 reaction) are plotted on the X-axis versus their corresponding measured value on the Y-axis calibrated using the wildtype sequence (ConcatP) as standard (N1 reaction). Black line shows wild type quantification and a perfect match between measured and true values. For all targets with mutations, quantification is underestimated, which is especially pronounced for BA.5.2 (green) and BF.10 (blue) where the measured value is about half the true value. The measured values for BQ.1 (brown), and all Omicron (red) are about 25 % depressed.