TABLE 1.

Rescue of ts10 by NPH-II alleles containing alanine mutations in motifs II and III

NPH-II gene	Virus titer at 40°C (PFU/ml)	Sp activity of protein (% of wild-type activity)
		ATPase	Helicase
Wild type	1 × 107	100	100
D296A	2 × 104	5a	<5a
E297A	3 × 104	5a	<5a
H299A	3 × 104	58	<1
E300A	1 × 104	1	<1
T326A	1 × 104	51	<1
T328A	1 × 104	52	1
Plasmid	<10

^aFrom reference 8.