TABLE 2.
Rescue of ts18 by NPH-II alleles containing alanine mutations in motifs I and Ia
| NPH-II gene | Virus titer at 40°C (PFU/ml) | Sp activity of protein (% of wild-type activity)
|
|
|---|---|---|---|
| ATPase | Helicase | ||
| Wild type | 2 × 107 | 100 | 100 |
| T187A | 3 × 106 | 68 | 52 |
| T192A | 4 × 104 | <0.2 | <1 |
| Q194A | 2 × 106 | 55 | 46 |
| P228A | 8 × 105 | 91 | 21 |
| R229A | 2 × 104 | 2 | <1 |
| Plasmid | <10 | ||