FIG. 2.

Southern blot analysis of DNA extracted from Akata cells harboring wild-type episomes (W), a parental clone of Akata harboring a mixture of wild-type and recombinant episomes (W/R), and two clones harboring pure recombinant episomes, derived either by infection of EBV-negative Akata cells (clone 2) (R) or by treatment of the parental clone with hydroxyurea (clone 26) (R/Hy). DNA was digested with XhoI, and the two identical halves of the membrane were cut apart and probed with either the EcoRI fragment (bp 125316 to 137221) of EBV (A) or the XmnI-HincII fragment containing the neomycin resistance gene (B). Sizes (in kilobases) are indicated by the arrows.