FIG. 5.

Induction of EBNA1 in SVKCR2 cells and the AGS gastric carcinoma cell line. (A and B) SVKCR2 cells were mock infected (Un) or infected with either wild-type virus (W) or one of two different clones (one in panel A and the other in panel B) of recombinant virus lacking gp150 (R) at the dilutions shown above the gels. The starting stocks of the viruses were adjusted so that they contained equal amounts of virus DNA. Samples of uninduced Akata cells (Ak) were included to demonstrate the mobility of EBNA1 in this strain. (C) AGS cells were mock infected (Un) or infected with equal amounts of recombinant virus (R) or wild-type virus (W). In all cases, 5 days after infection, cells were harvested for Western blotting with human serum containing antibodies to EBNA1.