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. 2021 Sep 22;33(8):637–654. doi: 10.1093/hmg/ddab216

Primers for qPCR (all are mouse specific).

Primer name Sequence
Myh6 (α-MHC) Forward CGGAACAAGACAACCTCAAT
Reverse TGGCAATGATTTCATCCAGC
Myh7 (β-MHC) Forward TTGCTACCCTCAGGTGGCT
Reverse CCTTCTCAGACTTCCGCAGG
Cd36 Forward GGCCAAGCTATTGCGACAT
Reverse CAGATCCGAACACAGCGTAGA
Cpt1b Forward TGCCTTTACATCGTCTCCAA
Reverse AGACCCCGTAGCCATCATC
Acadm (MCAD) Forward CATTCCGGAAAGTTGCGGTGG
Reverse TAATGGCCGCCACATCAGAG
Slc2a4 (GLUT4) Forward TTATTGCAGCGCCTGAGTCT
Reverse CAATCACCTTCTGTGGGGCAT
Hk2 Forward CCGACTCGCCGCAACAAG
Reverse ACTCGCCATGTTCTGTCCCAT
Pfkfb1 Forward TTTCGCCCAGACAACATGGA
Reverse TCAAAAACCGCAACGTGACC
Mthfd2 Forward AGGTCCCAAGCCTTTGAGTT
Reverse GTAAGGGAGTGCCGTTGAAA
Psat1 Forward AGTGGAGCGCCAGAATAGAA
Reverse CTTCGGTTGTGACAGCGTTA
Phgdh Forward GACCCCATCATCTCTCCTGA
Reverse GCACACCTTTCTTGCACTGA
Atf2 Forward TGTCATTGTGGCTGATCAGACTC
Reverse GTGTTGCAAGAGGGGACAAATC
Atf3 Forward CCAGAATAAACACCTCTGCCATCG
Reverse CTTCAGCTCAGCATTCACACTCTC
Atf4 Forward CCACCATGGCGTATTAGAGG
Reverse GTCCGTTACAGCAACACTGC
Atf5 Forward CTGGGAACCCCTGTGGATTAAA
Reverse GCAGCGTGGAAGATTGTTCA
Slc7a5 Forward TGCAGCCATGACCCTAACAG
Reverse AACAATGGGGACAGACCAGG
Asns Forward TGGCTGCCTTTTATCAGGGG
Reverse CAGATGCCCGAACTGTCGTA
Fgf21 Forward ATGGAATGGATGAGATCTAGAGTTGG
Reverse TCTTGGTGGTCATCTGTGTAGAGG
Gdf15 Forward GAGCTACGGGGTCGCTTC
Reverse GGGACCCCAATCTCACCT
Clpp Forward CCAAGCACACCAAACAGAG
Reverse GGACCAGAACCTTGTCTAAGAT
Lonp1 Forward CTGTGTTCCCGCGCTTTATC
Reverse GCCAGTGACAATCATTCGCAA
Fbxo32 (Atrogin1) Forward ACCTGCTGGTGGAAAACATC
Reverse CTTCGTGTTCCTTGCACATC
Trim63 (MuRF1) Forward AAGGAGCGCCATGGGATACTG
Reverse GTCACGCACGATTTCCC
Tfrc Forward AGCCAGATCAGCATTCTCTAACT
Reverse GCCTTCATGTTATTGTCGGCAT
Actb (β-actin) Forward CAACACCCCAGCCATG
Reverse GTCACGCACGATTTCCC