Figure 2.

Effect of F. duncaniae I-4574 administration prior to influenza infection on body weight loss, lung viral load and inflammation, and IFN production in bronchoalveolar lavage fluids. (A) Body weight loss at 7 dpi (n = 5, Mock and n = 10-12, IAV)). (B) left panel, the number of infectious particles was determined using a TCID₅₀ assay. Data are expressed as the mean number of infectious virus particles per lung. The solid lines correspond to the median values. Right panel, quantification of viral M1 protein transcript level in the lung (qRT-PCR assay). Data are expressed as genome copy/μg RNA (n = 10-13). (C) Bronchoalveolar lavage fluids were collected on 2 dpi. Concentrations of IFN-α, IFN-β, and IFN-Λ2,3 are shown (n = 7-8). (B, F) mRNA copy numbers were quantified by RT-PCR (7 dpi). Data are expressed as the mean fold change relative to average gene expression in mock-infected animals (n = 10-13). (E) BALs were collected on 7 dpi and TNF-α and CCL-2 were quantified by ELISA (n = 7-8). (B–F) One of two representative experiments is shown. For all graphs, errors indicate mean ± SEM. Significant differences were determined using the two-tailed Mann Whitney U test (B, D, F) and One-way ANOVA Kruskal-Wallis test (nonparametric), followed by the Dunn’s posttest test (A, C, E) (* P < 0.05, ** P < 0.01, *** P < 0.001).