Figure 4.

Effect of live and pasteurized F. duncaniae pre-treatment on body weight loss, lung viral load and inflammation. Mice received PBS-glycerol or A2-65 in PBS-glycerol 5 days before IAV infection and until the sacrifice. (A), Body weight loss during infection (expressed as percentage initial body weight). The right panel shows differences at 7 dpi. (B), the number of infectious particles was determined in a TCID₅₀ assay. The data are expressed as the mean number of infectious virus particles per lung. The solid lines correspond to the median values. (C), mRNA copy numbers in lungs were quantified by RT-PCR (7 dpi). The data are expressed as the mean fold change relative to average gene expression in mock-infected animals ± SEM. (D), Colon length measured at 7 dpi. (E), mRNA copy numbers in colons were quantified by RT-PCR (7 dpi). One of two representative experiments is shown (n = 6-8). For all graphs, errors indicate mean ± SEM. Significant differences were determined using One-way ANOVA Kruskal-Wallis test (nonparametric), followed by the Dunn’s posttest test (* P < 0.05, ** P < 0.01, *** P < 0.001).