FIG. 8.

RANTES (A) and MIP-1α (B) production by eosinophils cultured with purified RSV. Eosinophils were infected with RSV at an MOI of 10 (E+RSV) or incubated with culture medium (E). After 16 h, the supernatants were collected for RANTES and MIP-1α determination by ELISA. For the measurement of total content of RANTES and MIP-1α, eosinophils were lysed by Triton X-100 (E max). The results are expressed as mean ± SD of five experiments using eosinophil preparations from different donors. ∗, P < 0.01 compared with eosinophils cultured with medium or lysed by Triton X-100.