FIG. 3.

LMB inhibition of eGFP/ERev shuttling visualized by fluorescence microscopy. An eGFP/ERev fusion protein construct was transfected into 3T3 cells. Eighteen hours posttransfection the medium was replaced with medium containing 5 nM LMB and the cells were placed under the fluorescence microscope. eGFP fluorescence was recorded over several hours. The time, in minutes post-LMB addition, is shown in the lower right corner of each image. At 30 min a noticeable difference in subcellular eGFP/ERev localization is apparent. By 55 min the localization of the eGFP/ERev protein is almost completely nuclear.