FIG. 5.

Dose response curve of LMB effects on Rev-dependent and PRE-mediated gene expression in the CAT assay. pDM128 (RRE-containing RNA) and pRSVRev (▴), 138PRE (PRE-containing RNA) (■), and pDM138 (empty vector) (•) were transfected in the presence of increasing concentrations of LMB. Rev-dependent CAT expression decreased until it was maximally inhibited at 2 nM LMB. PRE-mediated CAT expression was not affected between 0 and 2 nM LMB and increased between 2 and 15 nM LMB. pDM138 CAT expression was not affected by LMB.