FIG. 6.

Northern blot analysis of nuclear and cytoplasmic RRE- and PRE-containing RNA in the presence or absence of LMB. Nuclear (lanes 1 to 6) and cytoplasmic (lanes 7 to 12) RNA of pDM138 (empty vector), pDM128 (RRE-containing RNA) and pRSVRev, and 138PRE (PRE-containing RNA) was transfected in the presence or absence of LMB. LMB inhibited Rev-induced cytoplasmic accumulation of RRE-containing RNA (compare lanes 9 and 10). Cytoplasmic accumulation of PRE-containing RNA was not affected by LMB (compare lanes 11 and 12). LMB had no effect on pDM138 CAT expression.