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Illustration of online sample generation and separation. (A) The valve is at the loading position, the droplet samples are drawn from the well plate and segmented by perfluorodecalin oil (yellow) in the perfluoroalkoxy alkane (PFA) tubing by sampling alternately from PFD and sample wells and sent to the internal sample groove in the valve. (B) The valve is at the injection mode. The internal sample groove loaded with a sample is sent to the left side for separation.
