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. Author manuscript; available in PMC: 2025 Mar 19.
Published in final edited form as: Anal Chem. 2024 Mar 5;96(11):4693–4701. doi: 10.1021/acs.analchem.4c00150

Figure 3.

Figure 3.

High-throughput fast separation tests with standard samples. (A) Top: 96 injections of samples infused in a continuous flow from the syringe. Bottom: zoomed-in of six separations from 129 to 154 s. (B) Top: 96 injections of samples drawn from the well plate as segmented droplets of alternating concentrations. Bottom: zoomed-in view of six separations from 235 to 265 s. (C) First, middle, and last separations in (A) overlaid. (D) First, middle, and last separations in (B) overlaid. For both experiments, the mobile phase was 35/65 acetonitrile and water +0.1% TFA. Flow rate was 70 μL/min. The analytes were 13.3 mM thiourea, 6.7 mM 2,5-dihydroxybenzoic acid, and 16.7 mM phenylacetic acid or its two-fold dilution dissolved in aqueous solutions with 20% acetonitrile. The wavelength for UV detection was 214 nm.