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3-MA aggravated the cell viability and apoptosis induced by melittin. (a) Cells were pretreated with 3-MA for 2 h and then treated with melittin (4 μg/ml) for 24 h. MTT assay was used to detect cell viability. (b) Detection of apoptosis. (c) Statistics of PI-positive cells. (d) Statistics of Annexin V-FITC-positive cells. *p < 0.05, **p < 0. 01, ***p < 0.001 versus the Control. #p < 0.05, ##p < 0. 01 versus the Melittin group.
