Figure 1. Description of the mold and seeding procedure.

(A) Micromachined stainless steel mold used to shape the gel. Design (left) and scanning electron microscopy image (right) of the mold. Scale bar: 500 µm. (B) Molded polyethylene glycol (PEG) gel (left – scale bar: 2 mm) and zoomed view of a pillar from its polydimethylsiloxane (PDMS) replica (middle – scale bar: 100 µm). Design and size of the pillar (right). (C) Timeline of the seeding procedure. (D) Cardiac rings in a well 1 day after seeding. Images stitched with ImageJ plugin. Scale bar: 1 mm. (E) Representative compaction of a ring with time after seeding (from day 0 to day 14), in brightfield. Scale bars: 200 µm.

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Figure 1C was created using BioRender, and is published under a CC BY-NC-ND 4.0. Further reproductions must adhere to the terms of this license.

Figure 1—figure supplement 1. Measurement of the Young’s modulus of polyethylene glycol (PEG) gel.

(A) Procedure to measure the Young’s modulus of a PEG gel disk by pipette aspiration. (B) Young’s modulus of 5% PEG gels (n=44). Mean ± SD.

Figure 1—figure supplement 2. Measurement of the efficiency of differentiation the induced pluripotent stem cells (iPSCs) into cardiomyocytes.

Percentage of positive cells for troponin T2 (TNNT2) determined by flow cytometry in the six different differentiations of wild-type (WT) iPSCs, used for these experiments. Mean ± SD.

Figure 1—video 1. Multiple rings beating at D14 – brightfield imaging ×4 magnification.

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