Figure 3. Contractility analysis of the tissues.

(A) Principle of the in-house Matlab code used for contractility analysis: detection of the central pillar and monitoring of the evolution of its area in time, calculation and plot of the strain ${ϵ}_A$ in time (ratio between the contraction amplitude in time and the maximum area of the central pillar). Representative plots of the strain in time and its derivative in time, for a tissue at day 14. (B–E) Evolution of beating parameters through time after seeding at days 1, 3, 7, 10, and 14. The changes of all the parameters through time are significant (p<0.0001 – ANOVA for repeated measures – D1: n=57, D3: n=59 D7: n=47, D10: n=43, D14: n=36 tissues, from three differentiations). Beating parameters at each time point are compared to their value at day 1. (B) Evolution of beat rate through time after seeding (***: p<0.002). (C) Evolution of contraction stress through time after seeding (****: p<0.0001). (D) Evolution of maximum contraction speed through time after seeding (****: p<0.0001). (E) Evolution of maximum relaxation speed through time after seeding (****: p<0.0001).

Figure 3—figure supplement 1. Strain ${ϵ}_A$ developed by the rings at D14.

Evolution of contraction strain ${ϵ}_A$ through time after seeding at days 1, 3, 7, 10, and 14. Contraction strain changes through time are significant (p<0.0001 – ANOVA for repeated measures – D1: n=57, D3: n=59 D7: n=47, D10: n=43, D14: n=36 tissues, from three differentiations). Contraction strain for each concentration is compared to contraction strain at day 1 (****: p<0.0001).

Figure 3—figure supplement 2. Contractile parameters of rings at D28.

Contractile parameters of rings at D28 expressed as a fold change to D14. Mann-Whitney test – D14: n=29 and D28: n=17, from two differentiations.

Figure 3—video 1. Contractility of a ring at day 14.

Download video file ^{(7.7MB, mp4)}