Figure 2. Production of C-terminally truncated Abl regulatory core constructs.

(A) Detailed view of the interaction site between the SH2 domain (yellow) and the C-terminus of the Abl KD with its αH- (dark blue) and αI- (cyan) helices from the crystal structure of the assembled Abl core (PDB 2FO0). The C^α-atoms of M515, Q517, and S519 are shown as cyan spheres (B) Top: western blot of E. coli-expressed, soluble truncated Abl constructs in the supernatant after cell lysis. Bottom: expression yields of the corresponding Abl constructs as quantified from the gel bands. The numbers on the horizontal axis denote the last amino acid of the respective construct Abl^83-X. (C) Relative yields of the purified truncated Abl constructs and Abl^83-534,E528K as quantified by OD₂₈₀.