Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Human) | Abl | Uniprot | P00519-2 | |
| Gene (Escherichia phage lambda) | Lambda phosphatase; LPP | Uniprot | P03772 | |
| Strain, strain background (Escherichia coli) | BL21(DE3) | Sigma-Aldrich | CMC0014 | Chemical competent cells |
| Recombinant DNA reagent | Plasmid containing human Abl83-534 |
Skora et al., 2013 DOI:10.1073/pnas.1314712110 |
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| Recombinant DNA reagent | Plasmid containing Lambda phosphatase |
Sonti et al., 2018 DOI:10.1021/jacs.7b12430 |
||
| Antibody | Conjugated poly-histidine antibody | Sigma-Aldrich | Cat.# A7058 | Mouse monoclonal (1:10000); peroxidase conjugate |
| Peptide, recombinant protein | Abltide | ProteoGenix | biotin-GGEAIYAAPFKK | |
| Commercial assay or kit | SAM2 Biotin Capture Membrane | Promega | Cat.# V2861 | |
| Chemical compound, drug | Asciminib; asci | Selleck Chemicals | Cat.# S8555 | |
| Chemical compound, drug | GNF-5 | Selleck Chemicals | Cat.# S7526 | |
| Chemical compound, drug | AMP-PNP | Roche | Cat.# 10102547001 | |
| Chemical compound, drug | Bosutinib; bosu | Selleck Chemicals | Cat.# S1014 | |
| Chemical compound, drug | Axitinib; axit | Selleck Chemicals | Cat.# S1005 | |
| Chemical compound, drug | Dasatinib; dasa | Selleck Chemicals | Cat.# S1021 | |
| Chemical compound, drug | Danusertib; danu | Selleck Chemicals | Cat.# S1107 | |
| Chemical compound, drug | Tozasertib; toza | Selleck Chemicals | Cat.# S1048 | |
| Chemical compound, drug | Staurosporine; staur | Selleck Chemicals | Cat.# S1421 | |
| Chemical compound, drug | PD180970 | Sigma-Aldrich | Cat.# PZ0142 | |
| Chemical compound, drug | PD166326 | Sigma-Aldrich | Cat.# PZ0366 | |
| Chemical compound, drug | Rebastinib; reba | Selleck Chemicals | Cat.# S2634 | |
| Chemical compound, drug | Ponatinib; pona | Selleck Chemicals | Cat.# S1490 | |
| Chemical compound, drug | Nilotinib; nilo | Selleck Chemicals | Cat.# S1033 | |
| Chemical compound, drug | Bafetinib; bafe | Selleck Chemicals | Cat.# S1369 | |
| Chemical compound, drug | Imatinib; ima | Selleck Chemicals | Cat.# S2475 | |
| Software, algorithm | NMRPipe |
Delaglio et al., 1995 DOI:10.1007/bf00197809 |
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| Software, algorithm | SPARKY |
Lee et al., 2015 DOI:10.1093/bioinformatics/btu830 |
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| Software, algorithm | NumPy |
Harris et al., 2020 DOI:10.1038/s41586-020-2649-2 |