Table 1. Michaelis-Menten parameters* for the various Abl helix constructs.
| Abl83-534 | Abl83-519 | Abl83-517 | Abl83-515 | Abl83-534•asciminib | Abl83-534,E528K | |
|---|---|---|---|---|---|---|
| vmax† | 89.4±6.7 | 79.5±15.4 | 56.8±13.3 | 3.2±0.2 | 61.9±11.8 | 201.7±16.0 |
| KM‡ | 51.8±8.8 | 91.4±30.9 | 74.8±35.7 | 39.5±9.0 | 336.7±80.3 | 71.6±12.3 |
| N§ | 8 | 3 | 3 | 3 | 3 | 2 |
*
the Michaelis-Menten parameters and their errors were obtained by Monte Carlo fitting using the sample mean and standard error of the mean of the measured kinase activities shown in Figure 5A.
†
in nmol Pi min–1μmol–1, where Pi is the transferred phosphate.
‡
in μM.
§
total number of activity assay experiments.