Figure 5. Intermittency encoded in olfactory sensory neuron (OSN) glomerular subpopulations.

(A) Color maps of glomerular intermittency binned by trial odor intermittency. Glomeruli are sorted by their glomerular intermittency (GI) slope (glomerular intermittency vs odor intermittency). Left: Example photoionization detector (PID) odor trace (red), raw sniff trace (black), and z-scored trace from one glomerulus. Horizontal line at y=2 indicates the threshold for glomerular intermittency quantification. (B) Left: Dendrogram for hierarchical cluster analysis. Gray indicates cluster 1 (37 glomeruli) and black indicates cluster 2 (191 glomeruli). Middle: Inter-glomerular correlation matrix. Colorbar corresponds to correlation coefficient (r) between two glomeruli (glomerular intermittency vs odor intermittency). Right: Colormap of normalized glomerular intermittency (normalized to individual glomerular maximum for clearer visualization of two clusters). Rows sorted by hierarchical clustering. (C) (i) Average slope of glomerular intermittency vs odor intermittency of cluster 1 and cluster 2 (μ_cluster1=–0.59±0.044, μ_cluster2=0.89±0.024). (ii) Average z-score response amplitude for glomeruli in cluster 1 and cluster 2 (μ_cluster1=6.3±0.55, μ_cluster2=7.5±0.33). (iii) Average T75 for glomeruli in cluster 1 and cluster 2 (μ_cluster1=179.2±5.6 ms, μ_cluster2=159.3±3 ms). (iv) Average correlation between glomerular deconvolved ΔF/F traces and PID reading for glomeruli in cluster 1 and cluster 2 (μ_cluster1=0.24±0.017, μ_cluster2=0.31±0.009). (D) Accuracy of linear classifier trained using 10, 15, 20, and 25 glomeruli (colorbar) at varying fractions of cluster 1 and cluster 2 glomeruli. (E) Left: Histogram of abs(GI slope) for all glomeruli. Black line indicates the bottom 25th percentile (0.53) and red line indicates the top 25th percentile (1.03). Right: True positive accuracy (CS+ predicted as CS+) of linear classifier trained on 0–50 glomeruli for glomeruli with the top 25th percentile of GI slopes (red) and the bottom 25th percentile of GI slopes. Dashed line indicates hit rate of animals on behavioral task (0.87).

Figure 5—figure supplement 1. Example clusters from three different animals.

Cluster 1 (white) and cluster 2 (red)-identified glomeruli in three different OMP-GCaMP6f animals.

Figure 5—figure supplement 2. Intermittency encoded in mitral and tufted (M/T) cell glomerular subpopulations.

(A) Left: Dendrogram for hierarchical cluster analysis. Gray indicates cluster 1 (34 glomeruli) and black indicates cluster 2 (161 glomeruli). Middle: Inter-glomerular correlation matrix. Colorbar corresponds to correlation coefficient (r) between two glomeruli (glomerular intermittency vs odor intermittency). Right: Color map of normalized glomerular intermittency. Rows sorted by hierarchical clustering. (B) (i) Average slope of glomerular intermittency vs odor intermittency of cluster 1 and cluster 2 (μ_cluster1=–0.49±0.04, μ_cluster2=1.17±0.04). (ii) Average z-score response amplitude for glomeruli in cluser 1 and cluster 2 (μ_cluster1=3.4±0.25, μ_cluster2=5.82±0.25). (iii) Average T75 for glomeruli in cluster 1 and cluster 2 (μ_cluster1=158.611.6 ms, μ_cluster2=144.4±2.5 ms). (iv) Average correlation between glomerular deconvolved ΔF/F traces and PID reading for glomeruli in cluster 1 and cluster 2 (μ_cluster1=0.16±0.005, μ_cluster2=0.27±0.007). (C) Accuracy of linear classifier trained using 10, 15, 20, and 25 glomeruli (colorbar) at varying fractions of cluster 1 and cluster 2 glomeruli. (D) Left: Histogram of abs(GI slope) for all glomeruli. Black line indicates the bottom 25th percentile (0.56) and red line indicates the top 25th percentile (1.29). Right: True positive accuracy (CS+ predicted as CS+) of linear classifier trained on 0–50 glomeruli for glomeruli with the top 25th percentile of GI slopes (red) and the bottom 25th percentile of GI slopes. Dashed line indicates hit rate of animals on behavioral task (0.85).