Figure 7. Effect of sniff frequency on glomerular representation of intermittency (2-heptanone).

For all graphs purple indicates olfactory sensory neurons (OSNs) (OMP-GCaMP6f) and green indicates mitral and tufted (M/T) cells (THY1-GCaMP6f). (A) Heatmap of glomerular intermittency across trials of 0.2, 0.5, and 0.8 odor intermittency values (colored heatmaps). Glomeruli are sorted based on their glomerular intermittency (GI) slope at 2 Hz. Gray bars next to heatmaps indicate the GI slope of each individual glomerulus. (B) % of intermittency encoding cells across sniff frequencies (OMP n=241 glomeruli, 6 mice; THY n=271 glomeruli, 6 mice). (C) GI slope as a function of sniff frequency. (D) GI slope at 8 Hz as a function of GI slope at 2 Hz. (E) Linear classifier performance (accuracy) over 240 glomeruli when trained on trials of four different sniff frequencies (2, 4, 6, 8 Hz). 60 iterations (20 times threefold) per classifier. Exponential plateau fit, OMP: 2 Hz: plateau = 0.95, Y=0.95–(0.44)*(e^–0.04x), r²=0.67; 8 Hz: plateau = 0.98, Y=0.98–(0.37)*(e^–0.04x), r²=0.7; THY: 2 Hz: plateau = 0.85, Y=0.85–(0.4)*(e^–0.03x), r²=0.54; 8 Hz: plateau = 0.94, Y=0.94–(0.4)*(e^–0.04x), r²=0.64.