Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Mtss1 (rabbit polyclonal) |
Novus Biologicals | Cat# NBP2-24716; RRID:AB_2716709 |
IHC (1:500) WB (1:500) |
| Antibody | Anti-Plexin-D1 (goat polyclonal) |
R&D Systems | Cat# AF4160; RRID:AB_2237261 |
IHC (1:500) |
| Antibody | Anti-Tau (goat polyclonal) |
Santa Cruz | Cat# sc-1995; RRID:AB_632467 |
ICC (1:500) |
| Antibody | Anti-Neurofilament (mouse) |
Hybridoma Bank | Cat# 2H3; RRID:AB_ 531793 |
IHC (1:500) |
| Antibody | Anti-β-actin/HRP (rabbit monoclonal) |
Cell Signaling Technology | Cat# 5125S; RRID:AB_1903890 |
WB (1:5000) |
| Antibody | Anti-Myc (mouse monoclonal) |
Cell Signaling Technology | Cat# 2276; RRID:AB_331783 |
WB (1:1000) |
| Antibody | Anti-Vsv (goat polyclonal) |
Abcam | Cat# ab3861; RRID:AB_304118 |
WB (1:1000) |
| Antibody | Anti-Sema3E (human polyclonal) |
LSBio | Cat# LS-c353198 | WB (1:500) |
| Antibody | Anti-Phospho-Akt (rabbit polyclonal) |
Cell Signaling Technology | Cat# 9271; RRID:AB_329825 |
WB (1:1000) |
| Antibody | Anti-Akt (rabbit polyclonal) |
Cell Signaling Technology | Cat# 9272; RRID:AB_ 329827 |
WB (1:1000) |
| Antibody | Anti-RFP (rabbit polyclonal) |
Abcam | Cat# ab62341; RRID:AB_945213 |
IHC (1:1000) |
| Antibody | Anti-RFP (mouse monoclonal) |
Thermo Fisher Scientific | Cat# MA5-15257; RRID:AB_10999796 |
IHC (1:1000) |
| Antibody | Anti-alpha-tubulin (mouse monoclonal) |
Sigma-Aldrich | Cat# T5168; RRID:AB_477579 |
IHC (1:1000) |
| Antibody | Anti-cleaved caspase3 (rabbit polyclonal) |
Cell Signaling Technology | Cat# 9661; RRID:AB_2341188 |
IHC (1:1000) |
| Antibody | Anti-CD31 (rat monoclonal) |
BD Bioscience | Cat# 553370; RRID:AB_394816 |
IHC (1:500) |
| Antibody | Anti-digoxigenin-alkaline phosphatase (sheep polyclonal) |
Roche | Cat# 11093274910; RRID:AB_2313640 |
In situ (1:3000) |
| Antibody | Anti-mouse IgG/HRP (goat polyclonal) |
Thermo Fisher Scientific | Cat# 31430; RRID:AB_228307 |
WB (1:10,000) |
| Antibody | Donkey anti-rabbit IgG/HRP (rabbit polyclonal) |
Jackson Immuno Research |
Cat# 711-035-152; RRID:AB_10015282 |
WB (1:10,000) |
| Antibody | Donkey anti-goat IgG/HRP (goat polyclonal) |
Jackson Immuno Research |
Cat# 705-035-147; RRID:AB_2313587 |
WB (1:10,000) |
| Antibody | Donkey anti-rabbit IgG, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21206; RRID:AB_2535792 |
ICC (1:1000) IHC (1:1000) |
| Antibody | Donkey anti-mouse IgG, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21202; RRID:AB_141607 |
ICC (1:1000) IHC (1:1000) |
| Antibody | Donkey anti-goat IgG, Alexa Fluor 568 | Thermo Fisher Scientific | Cat# A-11057; RRID:AB_142581 |
ICC (1:1000) IHC (1:1000) |
| Antibody | Donkey anti-rabbit IgG, Alexa Fluor 568 | Thermo Fisher Scientific | Cat# A-10042; RRID:AB_2534017 |
ICC (1:1000) IHC (1:1000) |
| Antibody | Donkey anti-mouse IgG, Alexa Fluor 568 | Thermo Fisher Scientific | Cat# A-10037; RRID:AB_2757558 |
ICC (1:1000) IHC (1:1000) |
| Antibody | Donkey anti-mouse IgG, Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A-31571; RRID:AB_162542 |
ICC (1:1000) IHC (1:1000) |
| Chemical compound, drug | MK2206 | SelleckChem | Cat# S1078 | |
| Chemical compound, drug | TRIzolTM Reagent | Thermo Fisher Scientific | Cat# 15596026 | |
| Chemical compound, drug | RNasin Ribonuclease Inhibitor | Promega | Cat# N2115 | |
| Chemical compound, drug | Halt Protease and Phosphatase Inhibitor Cocktail | Thermo Fisher Scientific | Cat# 78444 | |
| Chemical compound, drug | SuperSignal West Pico PLUS Chemiluminescent Substrate |
Thermo Fisher Scientific | Cat# 34580 | |
| Chemical compound, drug | SuperSignal West Femto Maximum Sensitivity Substrate |
Thermo Fisher Scientific | Cat# 34096 | |
| Chemical compound, drug | ProLong Diamond Antifade Mountant with DAPI |
Thermo Fisher Scientific | Cat# P36962 | |
| Chemical compound, drug | Eukitt Quick-hardening mounting medium | Sigma-Aldrich | Cat# 03989 | |
| Chemical compound, drug | Alexa Fluor 488 Phalloidin | Thermo Fisher Scientific | Cat# A12379 | |
| Chemical compound, drug | Alexa Fluor 568 Phalloidin | Thermo Fisher Scientific | Cat# A12380 | |
| Chemical compound, drug | Alexa Fluor 647 Phalloidin | Thermo Fisher Scientific | Cat# A22287 | |
| Chemical compound, drug | Gibco DMEM, high glucose, pyruvate | Gibco | Cat# 11995-065 | |
| Chemical compound, drug | Penicillin-Streptomycin | HyClone | Cat# SV30010 | |
| Chemical compound, drug | Paraformaldehyde | Electron Microscopy Sciences | Cat# 19202 | |
| Chemical compound, drug | Poly-d-lysine hydrobromide | Sigma-Aldrich | Cat# P6407 | |
| Chemical compound, drug | Corning Laminin | Corning | Cat# 354232 | |
| Chemical compound, drug | NBT/BCIP Ready-to-Use Tablets | Roche | Cat# 11697471001 | |
| Chemical compound, drug | DiI (1.1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate) | Sigma-Aldrich | Cat# 468495 | |
| Commercial assay or kit | QuantiTect Reverse Transcription kit | QIAGEN | Cat# 205313 | |
| Commercial assay or kit | Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23225 | |
| Commercial assay or kit | Lipofectamine 2000 Transfection Reagent | Thermo Fisher Scientific | Cat# 11668019 | |
| Commercial assay or kit | Basic Nucleofector Kit | LONZA | Cat# VAPI-1003 | |
| Commercial assay or kit | FD Rapid GolgiStain Kit | FD Neurotechnologies Inc | Cat# PK401A | |
| Cell line (Homo sapiens) | HEK293T Kidney (embryo) |
ATCC | CRL-3216; RRID:CVCL_0063 |
|
| Cell line (Cercopithecus aethiops) | COS7 Kidney |
Korean Cell Line Bank | Cat# 21651; RRID:CVCL_0224 |
|
| Cell line (H. sapiens) | HUVEC Umbilical Vein Endothelial Cells |
Lonza | CC-2935; RRID:CVCL_2959 |
|
| Cell line (H. sapiens) | HCMEC/D3 Human temporal lobe microvessels |
Millipore | SCC066; RRID:CVCL_U985 | |
| Strains | Mouse: C57BL/6J | The Jackson Laboratory | Stock# 000664; RRID:IMSR_JAX:000664 |
|
| Strains | Mouse: Nestin-Cre |
The Jackson Laboratory | Stock# 003771; RRID:IMSR_JAX:003771 |
|
| Strains | Mouse: Tie2-Cre |
The Jackson Laboratory | Stock# 008863; RRID:IMSR_JAX:008863 |
|
| Strains | Mouse: Drd1a-tdTomato |
The Jackson Laboratory | Stock# 016204; RRID:IMSR_JAX:016204 |
|
| Strains | Mouse: Mtss1flox/+ | Center for Animal Resources and Development Database (CARD) under permission of Dr. Mineko Kengaku |
Card ID#2760 | |
| Strains | Mouse: Plxnd1flox/flox | Obtained from Dr. Chenghua Gu |
Kim et al., 2011 | |
| Strains | Mouse: Sema3e+/- | Obtained from Dr. Chenghua Gu |
Chauvet et al., 2007 | |
| Software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ij/ | |
| Software, algorithm | Prism 9 | GraphPad | https://www.graphpad.com/scientific-Software/prism/ | |
| Software, algorithm | Image Lab (v5.2.1) | Bio-Rad | https://www.bio-rad.com/ | |
| Software, algorithm | Fusion FX | Vilber | https://www.vilber.com/fusion-fx/ | |
| Software, algorithm | LightCycler480 (v1.5.1) | Roche | https://lifescience.roche.com/ | |
| Software, algorithm | Leica Application Suite X | Leica | https://www.leicamicrosystems.com/ | |
| Software, algorithm | NIS-Elements AR (v4.51.00) | Nickon | https://www.microscope.healthcare.nikon.com/ | |
| Software, algorithm | NIS-Elements (v4.50.00) | Nickon | https://www.microscope.healthcare.nikon.com/ | |
| Software, algorithm | AIVIA | Aivia, Inc | https://www.aivia-Software.com/ | |
| Other | Immobilon-P PVDF Membrane | Merck | Cat# IPVH00010 | |
| Other | RNA-seq (P5 mice, striatum) | Data and code availability section in this paper | GEO: GSE196558 |