Extended Data Fig. 5. Recruitment of active zone components to heterologous synapses (related to Fig. 4).
(a) Recruitment of Munc13-1 to HEK293 cells expressing Nlgn1. Left. Representative images. Right. Summary statistics. Sph1, synaptophysin-1. Number of cells/batches: Ctrl1, 101/3; qKO1, 80/3. (b) Recruitment of RBP2 (RIM-BP2) to HEK293 cells expressing Nlgn1. Left. Representative images. Right. Summary statistics. Sph1, synaptophysin-1. Number of cells/batches: Ctrl1, 78/3; qKO1, 69/3. (c) Recruitment of Bassoon to HEK293 cells expressing Nlgn1. Left. Representative images. Right. Summary statistics. Number of cells/batches: Ctrl1,108/3; qKO1, 99/3. Scale bars, 20 μm. Data represented as means ± SEM; *** p < 0.001.