Extended Data Fig. 7. Analysis of Liprin-α-mediated clustering of PTPσ and Neurexin in HeLa cells (related to Fig. 6).
(a) HeLa cells co-transfected with GFP-tagged Liprin-α3 wildtype or the Liprin-α3 W895Q mutant, and Flag-tagged PTPσ. Left. Representative images with inserts showing enlarged (4x) regions of interest, and quantified (thresholded) signal to the right. Right. Quantification of the PTPσ cluster area per cell. (b) HeLa cells co-transfected with HA-tagged Neurexin and GFP-tagged liprin-α3 wildtype or its mutant (W921A) with or without Cherry-tagged CASK. Left. Representative images with inserts showing enlarged (4x) regions of interest and quantified (thresholded) signal to the right. Right. Quantification of the Neurexin cluster area per cell. Scale bars, 20 μm. Data represented as means ± SEM (number of cells/batches: 30/3, per condition). ** p < 0.01, *** p < 0.001.