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. 2024 Apr 8;7:427. doi: 10.1038/s42003-024-06111-x

Fig. 9. Down-regulation of FTL induced decreased iron homeostasis and cellular senescence.

Fig. 9

a Correlations between the FTL expression level and the iron storage pathway (PW: 0000592) score of AT2 cell cluster. b Correlations between the FTL expression level and the cellular senescence (GO: 0090398) score of AT2 cell cluster. c RT-qPCR analysis of relatively mRNA expression levels of FTL in BEAS-2B cells after transfecting with siRNA, by ANOVA test (left), shown as the means ± SD. Western Blot (mid) and semi-quantitative analysis (right) of relatively protein expression of FTL in BEAS-2B cells after transfecting with siRNA, by ANOVA test, shown as the means ± SD. d The CCK8 assay showed the viability of BEAS-2B cells, by two-tailed t-test, shown as the means ± SD. e SA-β-gal activity was measured by X-gal staining, by two-tailed t-test, shown as the means ± SD. f Western Blot (left) and semi-quantitative analysis (right) of relatively protein expression of p21 in BEAS-2B cells after transfecting with siRNA, by two-tailed t-test, shown as the means ± SD. g Schematic drawing. The choice of ANOVA tests and two-tailed tests was based on the results of Shapiro-Wilk normality test. Dunnett tests were used to inter-group comparison. ***p < 0.001, ****p < 0.0001. For results in c and d, 5 replicates from different cell samples were used in each group. For results in (e, f), 3 replicates from different cell samples were used in each group.