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. 2024 Apr 8;14:8159. doi: 10.1038/s41598-024-58963-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic view of the flowbot ONE (left) and the deck set-up used for automation of the Illumina DNA Prep (right). Both the pre-PCR and post-PCR parts of the workflow use the same deck set-up, with I: 96-well coldplate compatible with 8-tube strips (shown in blue); II: filter tips (1–50 μl, shown in white); III: 96-well plate containing small-volume Illumina DNA Prep reagents (shown in orange); IV: magnetic deck compatible with 96-well plates (shown in green); V: filter tips (2–200 μl, shown in white); VI: filter tips (1–50 μl, shown in white); VII: 24-tube rack containing medium-volume Illumina DNA Prep reagents (shown in yellow); IX: filter tips (2–200 μl, shown in white); X: Four column reservoir containing large-volume Illumina DNA Prep reagents (shown in black); XI: 24-tube rack containing the final, sequence-ready library DNA (shown in yellow). Deck positions VIII and XII are not used for the automation of the Illumina DNA Prep protocol (shown in solid grey).