Table 1.
Comparison of DNA concentrations measured after DNA extraction and after performing the manual and automated LP workflows.
| ID | Species (Gram-stain) | DNA concentrations (ng/µl) | Factor differenceb | ||
|---|---|---|---|---|---|
| DNA extract | Manual LP | Automated LPa | |||
| 1 | A. baumannii (-) | 0.8 | 15.2 | 7.6 | 2.0 |
| 2 | S. aureus ( +) | 0.1 | 2.9 | 1.7 | 1.7 |
| 3 | S. aureus ( +) | 0.2 | 7.7 | 5.2 | 1.5 |
| 4 | P. aeruginosa (-) | 1.3 | 15.1 | 10.4 | 1.5 |
| 5 | K. pneumoniae (-) | 0.4 | 10.9 | 1.9 | 5.6 |
| 6 | E. faecalis ( +) | < 0.1 | 1.4 | 0.6 | 2.2 |
| 7 | S. maltophilia (-) | 1.2 | 16.9 | 9.4 | 1.8 |
| 8 | C. difficile ( +) | 1.2 | 16.5 | 13.0 | 1.3 |
| 9 | E. cloacae (-) | 0.5 | 11.6 |
11.4 13.1 |
1.0 0.9 |
| 10 | P. aeruginosa (-) | 2.4 | 6.9 |
7.1 6.3 |
1.0 1.1 |
| 11 | K. pneumoniae (-) | 0.5 | 9.0 |
12.0 11.4 |
0.8 0.8 |
| 12 | E. coli (-) | 0.7 | 15.8 |
10.3 11.7 |
1.5 1.4 |
| 13 | S. aureus ( +) | 0.3 | 16.0 |
9.3 8.3 |
1.7 1.9 |
| 14 | C. difficile ( +) | 1.2 | 11.7 |
18.1 19.1 |
0.6 0.6 |
| 15 | C. difficile ( +) | 1.7 | 17.6 |
16.1 14.1 |
1.1 1.2 |
| 16 | S. aureus ( +) | 1.1 | 13.2 |
8.7 9.4 |
1.5 1.4 |
LP library preparation, − Gram-negative bacteria, + Gram-positive bacteria.
aThe automated LPs were performed in three independent flowbot ONE runs, with sample 1–8 processed in the first run and samples 9–16 processed in the second and third run for reproducibility testing.
bThe factor differences were calculated by dividing the DNA concentrations measured after manual LP by the DNA concentrations of the libraries obtained with the automated workflow.