Formation of hetero-oligomers between LQA/CCG and ΔLQA/CCG envelope glycoproteins. Serum from an HIV-1-infected individual was used to precipitate lysates of 293T cells transfected with plasmids encoding LQA/CCG and ΔLQA/CCG envelope glycoproteins. In lane 2:1, plasmids expressing the LQA/CCG and ΔLQA/CCG envelope glycoproteins were transfected at a 2:1 ratio, while in lane 1:1, the LQA/CCG- and ΔLQA/CCG-expressing plasmids were transfected in equal amounts. In lane 1:1, αV3, the same cell lysates as those used for the experiment in lane 1:1 were used for precipitation by the anti-V3 loop antibody 110.4.