TABLE 1.
HIV-1 envelope glycoprotein mutants and phenotypes
| Mutant | Heptad position | Proteolytic processing | Higher-order formsa | Surface expressionb | |
|---|---|---|---|---|---|
| 569 | T/C | d | − | − | NDc |
| 583 | V/C | d | − | − | ND |
| 586–587 | YL/CC | ga | − | − | ND |
| 568–570 | LTV/GCC | de | − | − | ND |
| 571–574 | WGIK/GCCG | ga | − | − | − |
| 576–578 | LQA/CCG | de | − | + | + |
| 578–581 | ARIL/GCCG | ga | − | − | − |
| 582–584 | AVE/GCC | de | − | − | − |
| 583–585 | VER/CCG | de | − | − | − |
a
Higher-order forms of the 576–578 LQA/CCG mutant were observed after boiling for 3 min in the presence of 0.2 to 2% β-mercaptoethanol.
b
Surface expression was assayed by fluorescence-activated cell sorter analysis and by binding of radiolabeled F105 antibody.
c
ND, not determined.