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. 2024 Apr 8;10:167. doi: 10.1038/s41420-024-01939-y

Fig. 7. TM reduces the levels of neuropathology and α-syn in the brains of A53T α-syn mice.

Fig. 7

a Representative images of TUNEL (green) and DAPI (blue) in brainstem of TM-treated mice. Scale bar represents 25 μm. b Quantification of TUNEL+ cells in the brainstem regions in (a). n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. c Representative images of TUNEL (green) and DAPI (blue) in striatum of TM-treated mice. Scale bar represents 25 μm. d Quantification of TUNEL+ cells in the striatum regions in (c). n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. e Representative images of Nissl staining in brainstem of TM-treated mice. Scale bar represents 100 μm. Detail scale bar: 50 μm. f Quantification of Nissl+ cells in brainstem regions in (e) using IpWin32 software. n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. g TM and apoptosis-related proteins in TM-treated mouse brain homogenate were analyzed by western blotting. h Relative levels of TM, p53, Caspase9, C-caspase9, Caspase3, C-caspase3, Bax, Bcl-2 in (g) were quantified using Image J software. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns not significant.