Figure 1.

Annexin A2 can bind with plasminogen by fashioning a complex with S100A11. (A) Endogenous lysyl oxidase-like 4 (LOXL4), annexin A2, and S100A11 proteins in the indicated cell lines were detected using the Western blotting (WB) procedure. The running gel was also stained with Coomassie brilliant blue (CBB) as a sample control of proper loading. (B) We evaluated the invasion ability of MDA-MB-231 cells after the treatment with or without tranexamic acid (TXA) (1 mg/mL). (C) HEK293T cells were co-transfected with HA-tagged S100A11 and Myc-tagged ANXA2. After precipitation of the expressed products with the beads, bound foreign proteins were analyzed by WB using the HA or Myc antibody. (D) Prepared recombinant proteins (GST, GST-ANXA2, S100A10, and S100A11) from the Escherichia coli expression system were checked for their purity. (E) Paired blots of the indicated proteins [GST, GST-annexin A2, and S100A10 (upper) or S100A11 (lower)] were dot-blotted onto the nitrocellulose filter membranes in a duplicate manner. The blotted membranes were then incubated with either biotinylated plasminogen (bio-PLG) alone or a blend of bio-PLG and S100A10 (upper) or S100A11 (lower). After incubation was completed, the bound proteins were detected by anti-S100A10 or anti-S100A11 (left) or streptavidin–horseradish peroxidase (HRP) (right). (F) The schematic diagram resumed the results from panel (E) The red or blue circles correspond to those highlighted in the results in panel (E) Data from panel (B) are means ± SD, ***p < 0.001. The individual symbols (*)mean exploit of preparations of the purified recombinant proteins as their predicted sizes.