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Effect of CypA on in vitro assembly. Negative-stain electron micrographs corresponding to in vitro assembly of CA (A to C) or ΔMA-CA (D and E) in the presence of purified CypA at a CA-to-CypA molar ratio of 10:1 (A and D), 3:1 (B and E), or 1:1 (C). (D and E). Grids had been coated with purified CA-specific antibodies. The arrow in panel A identifies a ring-like structure approximately 30 nm in diameter; the asterisk denotes a large, nonstructured protein aggregate. Bar, 100 nm.
