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. 2024 Apr 4;12(4):e006781. doi: 10.1136/jitc-2023-006781

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© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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Pyroptosis induces a polyfunctional antitumor antigen agnostic T cell response (A) Quantification of the number of infiltrating CD8⁺ T cells in chimeric tumors 7 days after activation of apoptosis (N=5) or pyroptosis (N=5). Error bars, SD. P values were determined using one-way ANOVA with Bonferroni correction with significance indicated (*p<0.0083). (B) Quantification of the frequency of LCMV-gp33-specific CD8⁺ T cells in circulation after activation of pyroptosis (N=5) or apoptosis (N=5) in chimeric tumors. Errors, SD. P values were determined using one-way ANOVA with Bonferroni correction with significance indicated (*p<0.0083). (C) Pyroptosis elicits significantly higher frequency of antigen experienced CD44⁺ CD69⁺CD8⁺ T cells within the spleen (N=4). Error bars, SD. P values were determined using Student’s t test with significance indicated (****p<0.0001). (D) Percent specific lysis of MC-38-gp33 cells after coculture with pyroptosis (N=4) and apoptosis (N=4) stimulated CD8⁺ T cells. Error bars, SD. P values were determined using two-way ANOVA with Tukey HSD with significance indicated (****p<0.0001). (E–H) Analysis of IL-2, TNF-a, IFN-g and IL-2⁺+TNFa⁺ coexpressing CD8⁺ T cells after restimulation with a peptide cocktail (Adgpk and Rpl18 and gp33) (N=5). Error bars, SD. P values were determined using one-way ANOVA with Bonferroni correction with significance indicated (****p<0.0001, ***p<0.0002, **p<0.001). (I) Analysis of CD3⁺CD4⁺ FoxP3⁺ regulatory T cells isolated from apoptosis (N=5) and pyroptosis (N=5) exposed chimeric tumors. Error bars, SD. P values were determined using one-way ANOVA with Bonferroni correction with significance indicated (***p<0.0002). ANOVA, analysis of variance; HSD, honest significant difference.