FIG. 3.

RG501 affects frameshifting at multiple viral frameshift signals. (A) Proposed frameshifting signals from other retroviruses. The ribosomal frameshift signals are shown for HIV-2 gag-pol, SIV gag-pol, HTLV-I GP, and HTLV-I PP. (B) RG501 stimulates frameshifting at multiple frameshift signals. Translations were performed in the presence of RG501 as described in Materials and Methods. For each pair of RNAs representing the different viral frameshift signals, at a given RG501 concentration, the percent frameshifting was determined by comparing the light output derived from the FS RNA with the light output derived from the L17 RNA. The percent frameshifting for each RG501 concentration was then compared to the percent frameshifting for the same viral constructs in the absence of RG501 and expressed as a fold increase in frameshifting HIV-1 (◊), HIV-2 (□), SIV (⧫), HTLV-I GP (•), and HTLV-I PP (▪).