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. 2024 Mar 13;92(4):e00505-23. doi: 10.1128/iai.00505-23

Fig 2.

Fig 2

DinJ inhibits the activation of inflammasome in J774A.1 cells. (A) Evaluation of cytotoxicity in J774A.1 cells infected with ΔfliC, ΔfliCΔdinJ, ΔfliCΔdinJ::dinJ, or empty vector-complemented strain ΔfliCΔdinJ::pMMB207 at an MOI of 100 for 4 h. (B and C) Enzyme-linked immunosorbent assay (ELISA) analysis assessing the secretion of IL-1β (B) and IL-6 (C) in the infected cells. (D) Western blotting analysis of the cleavage of Caspase-1, secretion of IL-1β, and the expression levels of NLRP3, NLRC4, and ASC in J774A.1 cells. β-actin was used as the loading control. Statistical significance was determined at P values of <0.001 (***) using the Student’s t-test.