TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| Escherichia coli strains | ||
| X7213λpir | Host for π requiring plasmids, conjugal donor | (17) |
| Salmonella Enteritidis strains | ||
| C50336 | Wild type | China Medical Culture Collection Center, National Institute for the Control of Pharmaceutical and Biological |
| C50336ΔfliC | C50336, in-frame deletion in fliC (parent strain) | (17) |
| ΔfliCΔdinJ | ΔfliC, in-frame deletion in dinJ | This study |
| ΔfliCΔdinJ::dinJ | ΔfliCΔdinJ with pMMB207 expressing the dinJ gene, Cmr | This study |
| ΔfliCΔdinJ::Vector | ΔfliCΔdinJ with pMMB207, Cmr | This study |
| ΔfliC-pCX340 | ΔfliC with pCX340, Tetr | This study |
| ΔfliC-pCX340-dinJ | ΔfliC with pCX340 expressing the dinJ gene, Tetr | This study |
| Plasmids | ||
| pDM4 | Suicide vector, pir dependent, R6K, SacBR, Cmr | (45, 46) |
| pMMB207 | Expression vector, Cmr | (47) |
| pMMB207-dinJ | pMMB207 derivative containing dinJ, Cmr | This study |
| pCX340 | pBR322 derivative, cloning vector used to fuse effectors to TEM-1-β-lactamase, Tetr | (33, 48) |
| pCX340-dinJ | pCX340 derivative containing dinJ, Cmr | This study |