TABLE 1.

Analysis of mutations in the 54 to 68 region of HIV MA^a

Mutation	Gag VLP	MA-MA interaction
S54A	+	+
E55A	+	+
C57Sb	−	−
R58A	+	+
Q59A	+	+
I60A	+	+
L61A	+	+
Q63A	+	+
L64Ab	−	−
Q65A	+	+
P66A	+	+
S67A	+	+
L68A	+	+
R58A/Q65A	+	+
Q59A/P66A	+	+
L61A/L68A	−	−
Y79A	−	ND

^aMutations were analyzed for properties of Gag VLP formation and the ability to incorporate Env antigen and MA oligomerization. We found no intermediate results in these assays and no evidence by EM for altered structures associated with any of the particle-competent mutants characterized. +, positive result; −, negative result; ND, not done. 

^bData from Morikawa et al. (28).