Table 1. Media composition for crypt isolation and enteroid growth.

Refer to Van Dussen et al.⁴⁰ and Miyoshi et al.⁴¹ for detailed methods describing the preparation of L-WRN conditioned media.

Media	Reagent	Volume	Final Concentration
Tissue Washing Media		500 mL
	Advanced DMEM/F12	435 mL
	1 M HEPES	5.0 mL	10 mM
	200 mM L-glutamine	5.0 mL	2 mM
	10,000 U/mL penicillin/streptomycin	5.0 mL	100 U/mL
	Fetal bovine serum (Heat Inactivated)	50 mL	10%
L-WRN primary culture media		637.5 mL
	Advanced DMEM/F12	500 mL
	200 mM L-glutamine	6.25 mL	2 mM
	10,000 U/mL penicillin/streptomycin	6.25 mL	100 U/mL
	Fetal bovine serum (Heat Inactivated)	125 mL	20%
50% L-WRN conditioned media		1000 mL
	L-WRN conditioned media	500 mL	50%
	L-WRN primary culture media	500 mL	50%