Table 2.

Media composition for the NEC-on-a-chip model.

Media	Reagent	Volume	Final Concentration
Chip expansion media		25 mL
	50% L-WRN condition media	23.4 mL
	1 M HEPES	250 µL	10 mM
	10 mM Y-27632	25 µL	10 µM
	5 mM CHIR99021	25 µL	5 µM
	10 mM SB 431542	25 µL	10 µM
	1 µM human [Leu15]-gastrin I	250 µL	10 nM
	5 mM A83-01	2.5 µL	500 nM
	50x B-27 Supplement	500 µL	1x
	100x N-2 Supplement	250 µL	1x
	500 mM N-acetylcysteine	50 µL	1mM
	50 µg/mL murine epidermal growth factor (mEGF)	25 µL	50 ng/mL
	1M Nicotinamide	250 µL	10mM
Chip differentiation media		25 mL
	50% L-WRN condition media	23.4 mL
	1 M HEPES	250 µL	10 mM
	10 mM SB 431542	25 µL	10 µM
	1 µM human [Leu15]-gastrin I	250 µL	10 nM
	5 mM A83-01	2.5 µL	500 nM
	50x B-27 Supplement	500 µL	1x
	100x N-2 Supplement	250 µL	1x
	500 mM N-acetylcysteine	50 µL	1mM
	50 µg/mL murine epidermal growth factor (mEGF)	25 µL	50 ng/mL
	1M Nicotinamide	250 µL	10mM
HIMEC media		500 mL
	Complete human endothelial cell media with the kit
	Fetal bovine serum (Heat Inactivated)	25 mL	5%