Lung physiology was measured using a flexiVent small animal ventilator. Inspiratory capacity (IC) was measured at baseline (A, B) and airway resistance (Rn) was measured with increasing methacholine (MCh) concentrations (C, D). IC was reduced in both sexes with (eCig Nic(+)) and without (eCig Nic(−)) nicotine when compared with their respective controls. Rn increased in male mice at 50 mg/mL both in the presence and absence of nicotine in the vapor (C), while in females Rn only increased with nicotine coexposure at MCh 50 mg/mL. Data represent at least five animals per group; *p<0.05, **p<0.01, using one-way ANOVA (A, B) and two-way ANOVA (C, D) with Bonferroni correction. ANOVA, analysis of variance; eCig, e-cigarette; Nic, nicotine.