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Representative western blots for ACE-2 protein (A) with densitometric analysis (B) and mRNA (C) from lung tissues of male and female mice exposed to e-cigarette vapor (±nicotine). Lung ACE-2 immunostaining (D) after e-cigarette exposure in male (upper panel) and female (lower panel) mice. (E) ACE-2 optical density of lung tissue (D) was quantified. Data represent five animals per group; *p<0.05, **p<0.01, ***p<0.001, using one-way analysis of variance with Bonferroni correction.
