Fig. 3.

MFG-E8 deficiency hinders OPCs from differentiating into mature oligodendrocytes. A Representative immunostaining of CC1 and Olig2 in the corpus callosum in the WT sham, MFG-E8^−/− sham, WT BCAS at 2 months, and MFG-E8^−/− BCAS at 2 months after surgery. Nuclei are stained with DAPI. Scale bar: 50 μm. B The density of CC1⁺Olig2⁺ cells in the corpus callosum in the above four groups (n = 4 per group **P < 0.01 vs WT sham, ^####P < 0.0001 vs MFG-E8^−/− sham, ^&&P < 0.01 vs WT BCAS, unpaired t-test). C Western blots showing the expression of NG2, PLP1, and MBP in the corpus callosum with GAPDH as a loading control. D Densitometry analyses of NG2, PLP1, and MBP expression normalized to GAPDH (n = 3 per group; **P < 0.01 vs WT sham, ^##P < 0.01, ^###P < 0.001 vs MFG-E8^−/− sham, ^&&P < 0.01, ^&&&P < 0.001 vs WT BCAS, unpaired t-test. All data are presented as the mean ± SD).